Zika virus lateral flow assays using reverse transcription-loop-mediated isothermal amplification

Detection of Raspberry ringspot virus Using Reverse Transcription Loop-mediated Isothermal Amplification

Raspberry ringspot virus (RpRSV), which has not occurred in Japan, infects many plants such as raspberry, gooseberry, cherry, grapevine, and strawberry. Biological assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) have been introduced for the detection of RpRSV in postentry quarantine in Japan. We studied reverse transcription loop-me...

Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Zika virus (ZIKV) has emerged as a major global public health concern in the last two years due to its link as a causative agent of human birth defects. Its rapid expansion into the Western Hemisphere as well as the ability to be transmitted from mother to fetus, through sexual transmission and possibly through blood transfusions has increased the need for a rapid and expansive public health re...

Visual Detection of West Nile Virus Using Reverse Transcription Loop-Mediated Isothermal Amplification Combined with a Vertical Flow Visualization Strip

West Nile virus (WNV) causes a severe zoonosis, which can lead to a large number of casualties and considerable economic losses. A rapid and accurate identification method for WNV for use in field laboratories is urgently needed. Here, a method utilizing reverse transcription loop-mediated isothermal amplification combined with a vertical flow visualization strip (RT-LAMP-VF) was developed to d...

Accuracy of Reverse Transcription Loop-Mediated Isothermal Amplification Technique for Detecting Dengue Virus

In recent years, researchers have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) test as a sensitive and specific technique. But the results of these studies were conflicting. The aim of this meta-analysis was to assess the accuracy of RT-LAMP technique for detecting dengue virus. We systematically searched PUBMED, EMBASE, Web of Science, and the Cochrane Lib...

Rapid Detection of West Nile Virus for Loop-Mediated Isothermal Amplification Real-Time Reverse Transcription